G6795

Anti-Green Fluorescent Protein (GFP), N-terminal antibody, Mouse monoclonal

clone GSN24, purified from hybridoma cell culture

• Synonym(e): Anti-GFP
• UNSPSC-Code: 12352203
• NACRES: NA.56

Eigenschaften

• Biologische Quelle: mouse
• Konjugat: unconjugated
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: GSN24, monoclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen 27 kDa
• Konzentration: ~2 mg/mL
• Methode(n): western blot: 1-2 μg/mL using GFP fusion proteins expressed in extracts of transfected cells
• Isotyp: IgG1
• Versandbedingung: dry ice
• Lagertemp.: −20°C; 2-8°C
• Posttranslationale Modifikation Target: unmodified

Beschreibung

Allgemeine Beschreibung

Anti-Green Fluorescent Protein (GFP), N-terminal antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma GSN24 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice.

Spezifität

The antibody reacts specifically with GFP fusion proteins.

Anwendung

Anti-Green Fluorescent Protein (GFP), N-terminal antibody, Mouse monoclonal has been used in: western blotting, enzyme linked immunosorbent assay (ELISA), immunofluorescence labeling.

Biochem./physiol. Wirkung

Green Fluorescent Protein (GFP) is a unique tool in cellular and molecular biology research to monitor gene expression and protein localization in living cells. In the jellyfish, A. victoria, GFP transduces the excitation energy resulting from emission of blue light of the photoprotein aequorin and reemits it as green light.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Lagerung und Haltbarkeit

For continuous use, store at 2–8 °C for up to one month. For extended storage, store at –20 °C in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Solutions at working dilution should be discarded if not used within 12 hours.

Haftungsausschluss

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable