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EMS0001

Sigma-Aldrich

PNGase Fast

recombinant, expressed in E. coli

Synonym(e):

N-Glycosidase F, PNGase F, Peptide N-glycosidase

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About This Item

UNSPSC-Code:
41131616

Rekombinant

expressed in E. coli

Qualitätsniveau

200

Konjugat

(N-linked)

Qualität

Proteomics Grade

Form

ready-to-use solution

Versandbedingung

wet ice

Lagertemp.

2-8°C

Verwandte Kategorien

Allgemeine Beschreibung

Peptide-N-glycosidase F (PNGase F) belongs to an enzyme family, that are mainly used for the deglycosylation of N-linked glycans.

Anwendung

PNGase Fast may be used to immobilize in order to perform deglycosylation. It may also be used to immobilize onto methacrylate based monolithic support to release the N-linked carbohydrate moieties from glycoproteins.

Biochem./physiol. Wirkung

Peptide-N-glycosidase F (PNGase F) cleaves asparagine-linked high mannose, hybrid and complex oligosaccharides from glycoproteins. It can also deaminate the asparagine to aspartic acid. PNGase Fast enables complete and rapid deglycosylation of antibodies and immunoglobulin fusion proteins, as well as other glycoproteins, to be prepared for downstream chromatography or mass spectrometry analysis. PNGase Fast creates an optimized workflow, reducing processing time without compromising sensitivity or reproducibility.

Lagerklassenschlüssel

10 - Combustible liquids

Analysenzertifikate (COA)

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David A Fischler et al.
Journal of biomolecular techniques : JBT, 30(4), 58-63 (2019-10-11)
There are several methods, both chemical and enzymatic, to release N-linked glycans for structural characterization. One of the most common enzymatic release methods is the use of peptide:N-glycosidase F (PNGase F). A less expensive and quicker alternative has been reported
Kathleen L Plona et al.
JIMD reports, 60(1), 56-66 (2021-07-15)
Due to advances in sequencing technologies, identification of genetic variants is rapid. However, the functional consequences of most genomic variants remain unknown. Consequently, variants of uncertain significance (VUS) that appear in clinical DNA diagnostic reports lack sufficient data for interpretation.

Artikel

A Rapid, Streamlined Workflow for Glycan Sample Preparation and Analysis by LC-MS

The use of PNGase Fast denaturing buffer and enzyme yielded results similar to a conventional 20-hour protocol with overnight digest while reducing workflow time to about 1 hour with a 15-minute digest.

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