PP0201
Glycoprofile™ II, Enzymatic In-Solution N-Deglycosylation Kit
sufficient for 20 samples
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About This Item
UNSPSC-Code:
12352202
NACRES:
NA.26
Empfohlene Produkte
Qualitätsniveau
Lagertemp.
2-8°C
Allgemeine Beschreibung
Contains sufficient reagents for a minimum of 20 reactions when the sample size is between one to two mg of a typical glycoprotein.
Anwendung
GlycoProfile II Enzymatic In-Solution N-Deglycosylation Kit was used to study the effects of glycosylation of the N-acetyl-D-galactosamine (GalNAc)-specific lectin found in Schizophyllum commune.
GlycoProfile™ II has been optimized to provide a convenient and reproducible method to remove N-linked glycans from glycoproteins and is compatible with subsequent MALDI-TOF mass spectrometric analysis without interference from any of the reaction components.
Glycoprofile™ II, Enzymatic In-Solution N-Deglycosylation Kit has been used in the deglycosylation of Schizophyllum commune lectin, Arabidopsis mannanase, human umbilical vein endothelial cells (HUVEC) protein lysate and organic solute transporter protein, Ostβ.
Rechtliche Hinweise
GlycoProfile is a trademark of Sigma-Aldrich Co. LLC
Nur Kit-Komponenten
Produkt-Nr.
Beschreibung
- 10× Reaction Buffer 1 mL/vial
Kit-Komponenten auch einzeln erhältlich
Produkt-Nr.
Beschreibung
SDB
- P7367PNGase F from Elizabethkingia meningoseptica, BioReagent, ≥95% (SDS-PAGE), for proteomics 50 USDB
- R7884Ribonuclease B from bovine pancreas, BioReagent, ≥50 Kunitz units/mg protein, ≥80% (SDS-PAGE) .5 mgSDB
- O9882Octyl β-D-glucopyranoside, BioXtra, ≥98% (GC) 100 mgSDB
- M31482-Mercaptoethanol, for molecular biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration) .9 mLSDB
Signalwort
Danger
Gefahreneinstufungen
Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral
Zielorgane
Liver,Heart
Lagerklassenschlüssel
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
Analysenzertifikate (COA)
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Members of the protein disulfide isomerase (PDI)-C subfamily are chimeric proteins containing the thioredoxin (Trx) domain of PDIs, and the conserved N- and C-terminal Pfam domains of Erv41p/Erv46p-type cargo receptors. They are unique to plants and chromalveolates. The Arabidopsis genome
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beta-Subunit of the Ostalpha-Ostbeta organic solute transporter is required not only for heterodimerization and trafficking but also for function
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Biochimica et biophysica acta, 1760(3), 326-332 (2006-03-02)
An N-acetyl-D-galactosamine (GalNAc)-specific lectin was purified from the edible mushroom, Schizophyllum commune, using affinity chromatography on a porcine stomach mucin (PSM)-Sepharose 4B column. Under reducing and non-reducing conditions, SDS-polyacrylamide gel electrophoresis gave a major band of 31.5 kDa. The Schizophyllum
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