EPC

93120820, fish skin, Epithelial

• UNSPSC-Code: 41106514

Eigenschaften

• product name: EPC, from fish skin, 93120820
• Biologische Quelle: fish skin
• Wachstumsmodus: Adherent
• Karyotyp: 2n = 96
• Morphologie: Epithelial
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice

Beschreibung

Ursprung der Zelllinie

Fish epithelioma Fathead minnow Pimephales promelas

Beschreibung der Zelllinie

This cell line known as Epithelioma papulosum cyprini (EPC) was originally reported to be from Carp (Cyprinus carpio) epidermal herpes virus-induced hyperplastic lesions. More recently EPC was found to be derived from Fathead Minnow (Pimephales promelas) see Winton et al., 2010. This has been confirmed by DNA sequencing at ECACC. The cells support the growth of pancreatic necrosis virus and rhabdovirus.

DNA-Profil

Not specified

Nährmedium

EMEM (EBSS) + 2 mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 using 0.25% trypsin/EDTA; 5% CO₂; 20 °C. Fish cell lines detach easily during transit if the culture is too young. After resuscitation split cells 1:3 and culture for at least one week with 1-2 media changes before shipping.

Sonstige Hinweise

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