CHSE-214 Cell Line from salmon

91041114, fish embryo, Not specified

• UNSPSC-Code: 41106514
• Biologische Quelle: fish embryo
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Not specified
• Produkte: Not specified
• Rezeptoren: Not specified

Eigenschaften

• Produktbezeichnung: CHSE-214 Cell Line from salmon, 91041114, embryo
• Biologische Quelle: fish embryo
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Not specified
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Salmon embryo

Beschreibung der Zelllinie

Derived from a Chinook salmon (Oncorhynchus tshawytscha) embryo, susceptible to a wide range of fish viruses and in many instances replicate high titres.

Anwendung

CHSE-214 cell line has been used for the growth and titration of infectious pancreatic necrosis virus (IPNV) from samples. It has also been used to carry out cell infection assays to determine the effect of tropodithietic acid (TDA)-exposed isolates.

Nährmedium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6; i.e seeding at 1-4 x 10,000 cells per cm using 0.25% trypsin or trypsin/EDTA, cells detach after 5-10 minutes at room temperature. Grow cells in dark (e.g. wrapped in foil); 5% CO₂; 21°C. Freeze cells in 5% DMSO and 95% foetal bovine serum (FBS). Fish cell lines detach easily during transit if the culture is too young. For resuscitation seed flasks at 4-5 x 10,000 cells per cm and then split cells 1:3 to 1:4 and culture for at least one week with 1-2 media changes before shipping.

Sonstige Hinweise

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