Sf9 Cell Line from Spodoptera frugiperda pupal ovarian tissue

89070101

• Synonym(e): SF-9 Cells
• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: insect ovary
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Spherical
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Spodoptera frugiperda pupal ovarian tissue

Beschreibung der Zelllinie

Derived from pupal ovarian tissue of spodoptera frugiperda. The cells are highly susceptible to Baculovirus infection and are used in the production of protein products genetically manipulated into Baculovirus vector systems.

Anwendung

Sf9 cell line has been used for Baculovirus viral studies. It has also been used for in vitro kynurenine-3-monooxygenase (KMO) inhibition assay.

Nährmedium

TC100 + 2mM Glutamine. (The medium can be supplemented with 10% Foetal Bovine Serum (FBS) if required).

Subkultur-Routine

Maintain cultures between 3x100,000 to 1x1,000,000 cells/ml; 27°C. Gently resuspend cells in old culture medium by dislodging the cells by pipetting, or gentle agitation, and resuspend in fresh medium. Cells should stick to the culture flask. Freeze in 9% DMSO/91% FBS. Cultures may take up to 10 days to recover normal growth characteristics following cryopreservation and resuscitation.

Sonstige Hinweise

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