R4655

Anti-Rab27 (N-terminal) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(e): Anti-RAB27A, member RAS oncogene family
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 200
• Konjugat: unconjugated
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen ~25 kDa
• Speziesreaktivität: human, rat
• Konzentration: ~1.5 mg/mL
• Methode(n): indirect immunofluorescence: 5-10 μg/mL using rat NRK cells fixed and permeabilized with 4% paraformaldehyde followed by 0.4% saponin; western blot: 2-5 μg/mL using whole extract of human A431 cells
• UniProt-Hinterlegungsnummer: P51159
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... RAB27A(5873) ; rat ... Rab27a(50645)

Beschreibung

Allgemeine Beschreibung

Rab27 (Ras-related protein) is a member of the Rab family of small guanosine triphosphatases (GTPases). Rab27 is widely expressed in non-neuronal regulated secretory cells, including exocrine, endocrine, ovarian, and hematopoietic cells.

Rab27A (Ras-related protein) belongs to the Rab family of small GTPase proteins. It is located on chromosome 15q21.

Immunogen

synthetic peptide corresponding to amino acid residues 45-57 of human RAB27A, with an added cysteine, conjugated to KLH. The corresponding sequence differs by one amino acid in rat and mouse Rab27A and by 2 amino acids in human RAB27B.

Anwendung

Anti-Rab27 (N-terminal) antibody has been used in immunoblotting, immunofluorescence.

Biochem./physiol. Wirkung

In humans, Rab27A (Ras-related protein) plays important roles in the development of cancers. It is considered as a prognostic biomarker for CRC (colorectal cancer) patients. It may participate in the transfer of protein and signal transduction mediated by GTPase. Rab27 mutation is linked with Griscelli syndrome type 2. It is also responsible for the innate immune resistance against invading microorganisms.

Rab27 (Ras-related protein) regulates the exocytosis of these cell-specific store organelles, using multiple organelle-specific effector proteins, such as granuphilin and melanophilin.

Physikalische Form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)