RTG-2

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 90102529, fish ovary, Fibroblast

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: fish ovary
• Verpackung: tube of 5 μg 90102529-DNA-5UG; pkg of vial of cells 90102529-1VL
• Wachstumsmodus: Adherent
• Karyotyp: 2n = 60
• Morphologie: Fibroblast
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Trout Rainbow gonad tissue

Beschreibung der Zelllinie

From pooled male/female gonad tissue of yearling rainbow trout (Oncorhynchus mykiss). First cell line to be established from cold-blooded vertebrates. 8-fold difference in growth rate in temperature range 4-24°C. Susceptible to infectious pancreatic necrosis virus, Egtved virus and arboviruses (EEE, VEE).

Anwendung

Virus studies: Inf. pacr. necrotic virus, Egtved virus, arboviruses (EEE, VEE)

Nährmedium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Passage using 0.25% trypsin below 24°C. Cells may be seeded as few as 3x10,000 cells/ml 5% CO₂; 24°C. Fish cell lines detach easily during transit if the culture is very youn

Sonstige Hinweise

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