HTC

93120108, rat liver, Epithelial

• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: HTC, 93120108
• Biologische Quelle: rat liver
• Wachstumsmodus: Adherent
• Karyotyp: 2n = 66, hypertriploid
• Morphologie: Epithelial
• Produkte: Not specified
• Rezeptoren: Dexamethasone phosphate; hydrocortisone
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Rat hepatoma

Beschreibung der Zelllinie

Derived from a rat ascites tumour, which in turn was derived from a solid hepatoma induced in male Buffalo rats by feeding them 0.04% N,N′-2,7fluorenyle-bis-2,2,2-trifluoroacetamide for 12.4 months.

Anwendung

Tumourigenicity and enzymatic studies

Nährmedium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.