AtT20

87021902, mouse brain, Small round cells growing as large aggregates

• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: AtT20, 87021902
• Biologische Quelle: mouse brain
• Wachstumsmodus: Aggregates in suspension
• Karyotyp: Diploid
• Morphologie: Small round cells growing as large aggregates
• Produkte: Adrenocorticotropic hormone (ACTH)
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse LAF1 pituitary gland tumour

Beschreibung der Zelllinie

Derived after alternate passage of LAF1 mouse pituitary tumour cells as tumours in animals and in cell culture. Cells produce adrenocorticotropic hormone (ACTH) at approximately the rate of 1000mU/mg of cell protein per week.

Anwendung

Hormone studies

Nährmedium

Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS). Cells grow in large aggregates.

Subkultur-Routine

Allow clusters to settle and remove all but 5 to 10 ml of medium. Gently transfer the clusters to new flasks and add fresh medium. A subcultivation ratio of 1:2 is recommended. Cells grow in large viable clusters making counting difficult. A seeding densi

Sonstige Hinweise

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