T98G Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 92090213, human brain, Not specified

• Synonym(e): T 98 G Cells, T-98G Cells, T98-G Cells
• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: human brain
• Beschreibung: Human Caucasian glioblastoma
• Verpackung: tube of 5 μg 92090213-DNA-5UG; pkg of vial of cells 92090213-1VL
• Wachstumsmodus: Adherent
• Karyotyp: Hyperpentaploid, modal no. 128-132
• Morphologie: Not specified
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human Caucasian glioblastoma

Beschreibung der Zelllinie

Derived from glioblastoma multiform tumour from a 61-year-old Caucasian male. Indefinite lifespan and anchorage independent but can enter viable G1 arrested state when deprived of serum.

Anwendung

Studies on the mechanism for cessation of proliferation in quiescent cells and cells synchronised in G1 phase.

T98G has been used to study the effects of the lysosomal destabilizing drug Siramesine on glioblastoma. It has also been used to study the efficiency of empty spiracles homeobox 2 (EMX2) in suppressing glioblastoma.

DNA-Profil

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 13
D16S539: 13
D5S818: 10,12
D7S820: 9,10
THO1: 7,9.3
TPOX: 8
vWA: 17,20

Nährmedium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1% Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Cells may become anchorage-independent if deprived of serum.

Sonstige Hinweise

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