C6 Cell Line from rat

92090409, rat nerve (glial tumor), Neuronal

• Synonym(e): C-6 Cells, RGC-6 Cells
• UNSPSC-Code: 41106514

Eigenschaften

• product name: C6 Cell Line from rat, 92090409
• Biologische Quelle: rat nerve (glial tumor)
• Beschreibung: Rat glial tumor
• Wachstumsmodus: Adherent
• Karyotyp: 2n = 42
• Morphologie: Neuronal
• Produkte: S-100 protein, somatotrophin
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Rat glial tumour

Beschreibung der Zelllinie

Derived from a rat glial tumour induced by N-nitrosomethylurea. The cells produce S-100 protein which is found in the neural tissue of vertebrates and has been found in brain tumours. Synthesis of S-100 increases 10 fold as cells reach confluency.

Anwendung

C6 cell line has been used to study the ability of 2-(2,4-dihydroxyphenyl)thieno-1,3-thiazin-4-one (BChTT) to inhibit cancer cell proliferation and identify mechanisms involved in its actions. It has also been used to study the cytotoxic and antioxidant properties of supercritical CO₂ extract (SCE) of old man′s beard (Usnea barbata).

C6 cells are reported to produce elevated levels of glyceryl phosphate dehydrogenase activity in response to glucocorticoids

Nährmedium

Ham′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x100,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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