HCT 116 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 91091005, human colon, Epithelial-like

• Synonym(e): HCT116 Cells
• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: human colon
• Verpackung: tube of 5 μg 91091005-DNA-5UG; pkg of vial of cells 91091005-1VL
• Wachstumsmodus: Adherent
• Karyotyp: Modal no. 45
• Morphologie: Epithelial-like
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human colon carcinoma

Beschreibung der Zelllinie

One of 3 strains of malignant cells isolated from a male with colonic carcinoma. Cells are tumourigenic in nude mice and form colonies on agarose. Growth and plating efficiency are enhanced by using a feeder layer of murine fibroblasts.

Anwendung

HCT 116 Cell Line has been used to:

• study the importance of cyclin D1 for the activity of lithocholic acid hydroxyamide (LCAHA)
• study the effects of different amounts of transferrin within the polyethyleneimine (PEI)-small interfering RNA (siRNA) complexes on the uptake of fluorescently-labelled siRNA
• determine the effect of PTS (polyamine transport system) in the selective uptake of Quilamine HQ1-44, an iron chelator

Tumourigenicity studies

DNA-Profil

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 7,10
D13S317: 10,12
D16S539: 11,13
D5S818: 10,11
D7S820: 11,12
THO1: 8,9
TPOX: 8
vWA: 17,22

Nährmedium

McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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