MCF7 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 86012803, human breast (adenocarcinoma), Epithelial-like

• Synonym(e): IBMF-7 Cells, MCF7 Cells, Michigan Cancer Foundation-7 Cells, ssMCF-7 Cells
• UNSPSC-Code: 41106514
• eCl@ss: 32190102

Eigenschaften

• Biologische Quelle: human breast (adenocarcinoma)
• Verpackung: tube of 5 μg 86012803-DNA-5UG; pkg of vial of cells 86012803-1VL
• Wachstumsmodus: Adherent
• Karyotyp: 2n = 46, hypertriploid to hypotetraploid
• Morphologie: Epithelial-like
• Produkte: Not specified
• Rezeptoren: Reported to express the oestrogen and progesterone receptor
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human Caucasian breast adenocarcinoma

Beschreibung der Zelllinie

Established from the pleural effusion from a 69 year female caucasian suffering from a breast adenocarcinoma. Cells exhibit some features of differentiated mammary epithelium including oestradiol synthesis and formation of domes. Cells may carry B or C type retrovirus and are considered to represent a category 2 pathogen (P2 containment). Cells express both the wild type and variant oestrogen receptors as well as progesterone receptor. The cells should be handled under laboratory containment level 2.

Anwendung

Tumourigenicity and virus studies: Type B and C viruses

DNA-Profil

STR-PCR Data:
Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 11,12
D5S818: 11,12
D7S820: 8,9
THO1: 6
TPOX: 9,12
vWA: 14,15

Nährmedium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids NEAA + 10% Foetal Bovine Serum FBS / FCS.

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% Trypsin or Trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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