HT29 Cell Line human

91072201, human colon, Epithelial

• Synonym(e): HT29 Cells
• UNSPSC-Code: 41106514

Eigenschaften

• product name: HT29 Cell Line human, 91072201, Caucasian colon adenocarcinoma grade II
• Biologische Quelle: human colon
• Wachstumsmodus: Adherent
• Karyotyp: 2n = 46, hypertriploid
• Morphologie: Epithelial
• Produkte: Secretory component of Immunoglobulin A (IgA), Carcinoembryonic antigen (CEA)
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human Caucasian colon adenocarcinoma

Beschreibung der Zelllinie

Isolated from a primary tumour in a 44 year old Caucasian female. Forms a well-differentiated adenocarcinoma consistent with colony primary, grade I. Tumours also form in steroid treated hamsters. Has the following HLA profile A1,3; B12,17; Cw5.

Anwendung

HT29 Cell Line has been used to determine viral titres of human parechovirus. It has also been used to study the ability of Bifidobacterium and Lactobacillus strains to counteract the toxic effect of C. difficile LMG21717.

Tumourigenicity studies

DNA-Profil

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 11,12
D16S539: 11,12
D5S818: 11,12
D7S820: 10
THO1: 6,9
TPOX: 8,9
vWA: 17,19

Nährmedium

McCoy′s 5A + 2mM Glutamine + 10% Foetal Bovine Serum FBS / FCS.

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% Trypsin; 5% CO₂; 37°C.

Sonstige Hinweise

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