SW 1116

87071006, human colon, Epithelial

• UNSPSC-Code: 41106514

Eigenschaften

• product name: SW 1116, 87071006
• Biologische Quelle: human colon
• Wachstumsmodus: Adherent
• Karyotyp: 2n = 46, hypotriploid
• Morphologie: Epithelial
• Produkte: Carcinoembryonic antigen (CEA)
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human Caucasian colon adenocarcinoma

Beschreibung der Zelllinie

Derived from a grade II adenocarcinoma of the colon extending into the muscularis from a 73 year old Caucasian male. The cells exhibit brush borders and produce high levels of CEA.

Anwendung

Tumourigenicity studies

Nährmedium

L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-3x10,000 cells/cm² using 0.25% trypsin/EDTA; CO₂ is not needed. Medium change after 4-5 days. 37°C.

Sonstige Hinweise

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