LS180

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 87021202, human colon, Epithelial-like

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: human colon
• Verpackung: tube of 5 μg 87021202-DNA-5UG; pkg of vial of cells 87021202-1VL
• Wachstumsmodus: Adherent
• Karyotyp: Modal no. 45 (2n=46)
• Morphologie: Epithelial-like
• Produkte: Carcinoembryonic antigen (CEA), Mucin
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human Caucasian colon adenocarcinoma

Beschreibung der Zelllinie

Derived from a 58 year old female Caucasian with Dukes type B adenocarcinoma of the colon. The cells secrete CEA and mucin and are tumorigenic in nude mice. LS174T (Sigma Catalogue number. 87060401) was derived from the same patient. The cells have not been exposed to trypsin.

Anwendung

Tumourigenicity studies

Nährmedium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-5x10,000 cells/cm² by scraping; 5% CO₂; 37°C.

Sonstige Hinweise

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