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11685619910

Roche

Fluorescein RNA Labeling Mix

solution, suitable for Northern blotting, suitable for Southern blotting, suitable for hybridization

Synonym(e):

RNA labeling

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About This Item

UNSPSC-Code:
41105500

Form

solution

Qualitätsniveau

100

Verwendung

sufficient for 20 reactions

Verpackung

pkg of 40 μL

Hersteller/Markenname

Roche

Methode(n)

Northern blotting: suitable
Southern blotting: suitable
hybridization: suitable

Lagertemp.

−20°C

Verwandte Kategorien

Allgemeine Beschreibung

Fluorescein RNA Labeling Mix is a convenient nucleotide mixture for the labeling of RNA with fluorescein-12-uridine triphosphate (UTP).

Spezifität

Heat inactivation: Stop the reaction by adding 2μl 0.2M EDTA (pH 8.0).

Anwendung

Fluorescein-labeled RNA has been used in a variety of hybridization techniques such as:
  • in situ hybridization to tissues
  • Southern blots
  • northern blots
  • plaque or colony lifts
  • RNase protection experiments

Qualität

The mix is function tested in combination with the DIG RNA Labeling Kit.

Prinzip

Fluorescein-labeled, single-stranded RNA probes of defined length are generated by in vitro transcription. Fluorescein-12-UTP is incorporated by SP6, T7, and T3 RNA polymerases at approximately every 20 to 25th nucleotide of the transcript under standard conditions. The Fluorescein RNA Labeling Mix is specifically designed for use with the RNA polymerases SP6, T7, and T3, which are supplied with an optimized transcription buffer.

Angaben zur Herstellung

10x concentrated NTP labeling mixture: 10mM ATP, 10mM CTP, 10mM GTP, 6.5mM UTP, 3.5mM Fluorescein-12-UTP, pH 7.5 (+20°C).

Sonstige Hinweise

For life science research only. Not for use in diagnostic procedures.

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

nwg

Flammpunkt (°F)

No data available

Flammpunkt (°C)

No data available

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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