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10108090001
Roche
Peroxidase (POD)
grade I, from horseradish
Synonym(e):
POD, peroxidase
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About This Item
Empfohlene Produkte
Biologische Quelle
horseradish
Qualitätsniveau
Form
lyophilized (salt-free)
Spezifische Aktivität
≥250 units/mg protein (At 25 °C with guaiacol and H2O2 as the substrates.)
250 U/mg
Verpackung
pkg of 25,000 U
Hersteller/Markenname
Roche
Optimaler pH-Wert
6.0-6.5
Versandbedingung
wet ice
Lagertemp.
2-8°C
Allgemeine Beschreibung
Donor:hydrogen-peroxide oxidoreductase
Peroxidase (POD) (E.C. 1.11.1.7) is extensively expressed in most fruits and vegetables.
Anwendung
Oxidation of electron donors (e.g., epoxidation of double bonds). Peroxidase can be coupled to other proteins via its amino groups, as well as its carbohydrate moiety. The reconstituted solution can be used directly for conjugation without prior dialysis.It has been used in ligand blotting.
Peroxidase (POD) has been used in AUR (Amplex UltraRed assay) to measure extracellular H2O2 produced from disaggregated slug cells.
Biochem./physiol. Wirkung
Peroxidase (POD) is involved in the dehydrogenation of a large number of organic compounds including, phenols and aromatic amines, hydroquinones and hydroquinoid amines, mainly benzidine derivatives.
Qualität
Contaminants: <0.001% ATPase and acid phosphatase each, <0.7% catalase
Purity number: approximately 3.0 (A403/A275)
Purity number: approximately 3.0 (A403/A275)
Angaben zur Herstellung
Activator: Ammonia, pyridine, imidazole, all at pH >7; detergents.
Working solution: PBS (phosphate buffered saline) or water (starting concentration 20 mg/ml).
Working solution: PBS (phosphate buffered saline) or water (starting concentration 20 mg/ml).
Rekonstituierung
Solution (10 mg/ml in water): clear, red brown; pH-value: 6.0 to 7.0.
Sonstige Hinweise
For life science research only. Not for use in diagnostic procedures.
Signalwort
Danger
H-Sätze
Gefahreneinstufungen
Resp. Sens. 1 - Skin Sens. 1
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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