MABS2047M

Anti-MT-ND2 Antibody, clone 9E12-1B3

clone 9E12-1B3, from mouse

• Synonym(e): NADH-ubiquinone oxidoreductase chain 2, EC: 1.6.5.3, NADH dehydrogenase subunit 2, MT-ND2
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: 9E12-1B3, monoclonal
• Speziesreaktivität: human
• Verpackung: antibody small pack of 25 μg
• Methode(n): western blot: suitable
• Isotyp: IgG2aκ
• NCBI-Hinterlegungsnummer: YP_003024027.1
• UniProt-Hinterlegungsnummer: P03891
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... MT-ND2(4536)

Beschreibung

Allgemeine Beschreibung

NADH-ubiquinone oxidoreductase chain 2 (UniProt: P03891; also known as EC: 1.6.5.3, NADH dehydrogenase subunit 2, MT-ND2) is encoded by the MT-ND2 (also known as MTND2, NADH2, ND2) gene (Gene ID: 4536) in human. MT-ND2 is a mitochondrial inner membrane protein that forms the core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I). It is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (Coenzyme Q10) that participates in the generation of a proton gradient, which is then used for ATP synthesis. Defects in MT-ND2 gene are known to cause of Leber hereditary optic neuropathy (LHON) that is a maternally inherited disease resulting in acute or subacute loss of central vision, due to optic nerve dysfunction. LHON results from primary mitochondrial DNA mutations affecting the respiratory chain complexes.

Spezifität

Clone 9E12-1B3 detects NADH-ubiquinone oxidoreductase chain 2 (MT-ND2) in mitochondria from human cells. It targets an epitope within the N-terminal region.

Immunogen

Epitope: N-terminus

Synthetic peptide corresponding to the N-terminus of human MT-ND2.

Anwendung

Research Category
Zelluläre Signaltransduktion

Anti-MT-ND2, clone 9E12-1B3, Cat. No. MABS2047, is a mouse monoclonal antibody that detects NADH-ubiquinone oxidoreductase chain 2 and has been tested for use in Western Blotting.

Western Blotting Analysis: 2 µg/mL from a representative lot detected MT-ND2 in mitochondria from human neonatal dermal fibroblasts and mitochondria from human neonatal dermal fibroblasts depleted of mtDNA (Courtesy of Michael F. Marusich, Ph.D., mAbDx, Inc., Eugene, OR USA).

Qualität

Evaluated by Western Blotting in mitochondria from human neonatal dermal fibroblasts and mitochondria from human neonatal dermal fibroblasts depleted of mtDNA.

Western Blotting Analysis: 1 ug/mL of this antibody detected MT-ND2 in 10 µg of human neonatal dermal fibroblasts and mitochondria from human neonatal dermal fibroblasts depleted of mtDNA.

Zielbeschreibung

~30 kDa observed; 38.96 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physikalische Form

Protein L

Format: Purified

Purified mouse monoclonal antibody IgG2a in buffer containing HEPES-Buffered Saline (150 mM NaCl, 15 mM HEPES, pH 7.2) with 0.02% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable