23-024-M

p27 Protein complex, activated, 10 µg

Activated complex of three (3) full length, recombinant human proteins: p27, Cyclin E1, & CDK2. Phosphorylated in vitro using CDK2/Cyclin E1. For use in Enzyme Assays. Behaves as a substrate in ubiquitin assays.

• Synonym(e): p27 ^kip1, Cyclin-dependent kinase inhibitor 1B, Kip1
• UNSPSC-Code: 12352202
• eCl@ss: 32160405
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: human
• Qualitätsniveau: 100
• Rekombinant: expressed in baculovirus infected Sf21 cells
• Hersteller/Markenname: Upstate^®
• Methode(n): activity assay: suitable
• GenBank-Hinterlegungsnummer: BC001971 (p27)
• NCBI-Hinterlegungsnummer: NM_001238 (Cyclin E1); NM_001798
• Angaben zum Gen: human ... CDK2(1017) , CDKN1B(1027)

Beschreibung

Allgemeine Beschreibung

p27 is an important regulator of cell cycle progression, involved in G1 arrest. It is a potent inhibitor of cyclin E1- and cyclin A-CDK2 complexes and a positive regulator of cyclin D-dependent kinases such as CDK4. The degradation of this protein, which is triggered by its CDK-dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Decreased levels of p27 are found in various epithelial tumours originating from lung, breast, colon, ovary, oesophagus, thyroid and prostate. Defects in the gene encoding p27 (CDKN1B) are the cause of multiple endocrine neoplasia type 4 (MEN4) an inherited cancer of the thyroid.

Sonstige Hinweise

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Rechtliche Hinweise

GenBank is a registered trademark of United States Department of Health and Human Services

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: nwg
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable