L021000

DCA-Deblock

with Dichloromethane

• UNSPSC-Code: 12352200

Eigenschaften

• Qualitätsniveau: 200
• Typ: for DNA synthesis; for DNA synthesis
• Produktlinie: Proligo Reagents
• Form: liquid
• Lagerbedingungen: dry at room temperature
• Konzentration: 42.2-51.6 g/L (DCA content, titration)
• Verunreinigungen: ≤200 ppm water content (Karl Fischer)
• Farbe: clear

Beschreibung

Allgemeine Beschreibung

Deblock solution contains dichloroacetic acid (DCA) in dichloromethane that assists in the removal of the dimethoxytrityl (DMT) protecting group from the 5′hydroxyl moiety of nucleotides already incorporated into the growing nucleic acid, before the addition of the next phosphoramidite. Removal of the DMT allows the unprotected 5′hydroxyl moiety to react with a new phosphoramidite in a subsequent extension reaction.

Angaben zur Herstellung

Dicloromethane / Dichloroacetic acid 97/3 (v/v)

Sonstige Hinweise

Deblock solution containing dichloroacetic acid (DCA) in dichloromethane removes the dimethoxytrityl (DMT) protecting group from the 5′hydroxyl moiety of nucleotides already incorporated into the growing nucleic acid, prior to the addition of the next phosphoramidite. Removal of the DMT allows the unprotected 5′hydroxyl moiety to react with a new phosphoramidite in a subsequent extension reaction.

Sicherheitshinweise

• Piktogramme: GHS05,GHS07,GHS08
• Signalwort: Danger
• H-Sätze: H315 - H318 - H336 - H351 - H360FD - H362
• P-Sätze: P202 - P263 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Gefahreneinstufungen: Carc. 2 - Eye Dam. 1 - Lact. - Repr. 1B - Skin Irrit. 2 - STOT SE 3
• Zielorgane: Central nervous system
• Lagerklassenschlüssel: 6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• WGK: WGK 3