UHPLC analysis of Benzoyl peroxide and degradation products on Purospher® STAR RP-18e Hibar® HR
CONDITIONS
column
Purospher STAR RP-18e Hibar HR, 10 cm x 2.1 mm I.D., 2.0 μm, 120Å (1.50648.0001)
mobile phase
[A] 5 mM Potassium Phosphate, pH 3.0 with phosphoric acid in water; [B] Acetonitrile
gradient
40 to 80% B in 9 min; hold at 80% B for 2 min; to 40% B in 0.1 min; to hold at 40% B for 6.9 min
flow rate
0.3 mL/min
pressure
4236 psi (292 bar)
column temp.
30 °C
detector
UV, 235 nm
injection
1 μL
sample
100 μg/mL Benzoic Acid, Ethyl Benzoate, Benzoyl Peroxide and Benzaldehyde in 30:70 acetonitrile:5mM potassium phosphate pH 3.0 in water with phosphoric acid
Description
Analysis Note
Benzoyl peroxide is used as an acne treatment, for bleaching flour, hair and teeth, for cross-linking polyester resins, and for many other purposes. Benzoyl peroxide is split into benzoic acid and oxygen when it contacts the skin breaking down the keartin in the outermost layer, as well as aiding in the cleaning of sebum and cellular debris from pores. This helps to lower bacterial (antibacterial effect) populations in the pores on the skin. It also has a mild drying effect, which allows excess oils and dirt to be easily washed away from the skin.
Legal Information
Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany