HPLC Analysis of Lutein on Ascentis® Express C18
CONDITIONS
sample preparation
SPE (Solid Phase Extraction)
column
Ascentis Express C18, 5 cm × 2.1 mm I.D., 2.7 μm particles (53822-U)
mobile phase
[A] Acetonitrile; [B] Water; (90:10, A:B)
flow rate
1.0 mL/min
pressure
2581 psi (178 bar)
column temp.
30 °C
detector
UV, 450 nm
injection
0.3 μL
sample
0.10 mg/mL of Lutein in 50/50 MeOH/THF
Description
Analysis Note
The analysis of lutein is relevant to pharmaceutical, nutraceutical, food, pet foods, and animal and fish feed applications. This method uses a 2.7 μm Ascentis® Express C18 column for a rapid analysis of lutein in under two minutes with UV detection.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany