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HPLC Analysis of Lutein on Ascentis® Express C18

HPLC Analysis of Lutein on Ascentis® Express C18 application for HPLC

Materials

Analytical column

Product No.
Description
Pricing

53822-U

Ascentis® Express C18, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 5 cm × 2.1 mm

Standard

Product No.
Description
Pricing

07168

Lutein

analytical standard

CONDITIONS

sample preparation

SPE (Solid Phase Extraction)

column

Ascentis Express C18, 5 cm × 2.1 mm I.D., 2.7 μm particles (53822-U)

mobile phase

[A] Acetonitrile; [B] Water; (90:10, A:B)

flow rate

1.0 mL/min

pressure

2581 psi (178 bar)

column temp.

30 °C

detector

UV, 450 nm

injection

0.3 μL

sample

0.10 mg/mL of Lutein in 50/50 MeOH/THF

Description

Analysis Note

The analysis of lutein is relevant to pharmaceutical, nutraceutical, food, pet foods, and animal and fish feed applications. This method uses a 2.7 μm Ascentis® Express C18 column for a rapid analysis of lutein in under two minutes with UV detection.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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Small Molecule HPLCLarge Molecule HPLCGas Chromatography (GC)TLCSolid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS