Skip to Content
MilliporeSigma

HPLC Analysis of Levothyroxine and Liothyronine on Ascentis® Express ES-Cyano

HPLC Analysis of Levothyroxine and Liothyronine on Ascentis® Express ES-Cyano application for HPLC

Materials

analytical column

Product No.
Description
Pricing

53491-U

Ascentis® Express ES-Cyano, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 10 cm × 4.6 mm

standard

Product No.
Description
Pricing

T6397

3,3′,5-Triiodo-L-thyronine sodium salt

powder, BioReagent, suitable for cell culture

CONDITIONS

column

Ascentis Express ES-Cyano, 10 cm x 2.1 mm I.D., 2.7 μm particles (53491-U)

mobile phase

[A] water with 0.05% phosphoric acid; [B] acetonitrile with 0.05% phophoric acid; (60:40, A:B)

flow rate

1.5 mL/min

pressure

3270 psi (225 bar)

column temp.

30 °C

detector

UV, 225 nm

injection

5 μL

sample

20 μg/mL in mobile phase

Description

Analysis Note

This application demonstrates the suitabilty of Ascentis Express ES-Cyano for the analysis of Levothyroxine according to USP Monograph 3301 with Liothyronine as a reference standard.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Chromatogram Search

Related Product Categories

HPLC ColumnsGC Columns & AccessoriesTLC Plates & AdsorbentsSolid Phase Microextraction (SPME)Solid Phase Extraction & QuEChERS

Related Applications

Small Molecule HPLCLarge Molecule HPLCGas Chromatography (GC)TLCSolid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS