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Transfer Buffers & Reagents

Protein Transfer in Western Blotting

Protein transfer is a critical step in Western blotting techniques. Premixed transfer buffers offer consistent, highly reliable protein transfer in convenient ready-to-use options. High-quality electrophoresis-grade chemicals can also be used to make fresh transfer buffer in customizable volumes as needed. Specially formulated, high purity transfer buffer reagents and chemicals are available for optimal protein transfer to PVDF or nitrocellulose and superior Western blotting results.

Related Product Resources

Article: Western Blotting Protocol (Immunoblotting Protocol)
Protocol: Protein Blotting Handbook
Brochure: Rethink Western Blotting

Related Product Categories
Transfer Membranes & Kits
Choose from a variety of Immobilon® PVDF membranes for optimal Western blotting and protein immunoblotting performance based on target protein size, application, detection method, and convenience.
Protein Gel Electrophoresis and Transfer Equipment
Electrophoresis tanks, blotting systems, and power supplies for protein gel electrophoresis and wet and semi-dry transfer.
Protein Electrophoresis Gels & Buffers
Precast polyacrylamide protein gels, running buffers, and hand-casting reagents for hand-pouring polyacrylamide gels for PAGE and SDS-PAGE protein gel electrophoresis.
Protein Electrophoresis Gel Stains
High sensitivity colorimetric and fluorescent protein gel stains, fixing solutions, and de-staining reagents for polyacrylamide gels, agarose gels, and PVDF and nitrocellulose membranes.
Protein Markers
Pre-stained and unstained molecular weight markers for protein gel electrophoresis, SDS-PAGE, and Western blotting applications.
Membrane Immunodetection Devices
Simplify your Western blotting workflow and save time using immunodetection devices for Western blot blocking, antibody binding, and washing.
Immunodetection Reagents & Substrates
High quality blocking reagents, secondary antibodies, and HRP and AP substrates for Western blotting and immunoblotting detection.
Related Applications
Western Blotting
An overview of the principles and applications of Western blotting for protein detection. Includes links to detailed protocols, videos, and other resources for protein extraction, gel electrophoresis, transfer to PVDF or nitrocellulose membranes, and chemiluminescent, colorimetric, and fluorescent detection methods.
Gel Electrophoresis
Protein gel electrophoresis is used to separate proteins for purification, characterization, and detection. Methods such as native PAGE, SDS-PAGE, 2D PAGE, and isoelectric focusing (IEF) are used in preparation for downstream applications including Western blotting, mass spectrometry, and proteomic analysis.
Protein Purification
Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.
Protein Expression
Protein expression technologies for expressing recombinant proteins in E. coli, insect, yeast, and mammalian expression systems for fundamental research and the support of therapeutics and vaccine production.
Lysis & Protein Extraction
An overview of cell lysis and protein extraction methods including detergent solubilization, freeze-thaw lysis, osmotic shock, sonication, enzymatic cell lysis, and mechanical disruption techniques such as Dounce, Polytron, and mortar and pestle homogenization.
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