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Membrane Immunodetection Devices

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Western blotting immunodetection involves the use of antibodies to detect target protein. Blotted membranes containing transferred proteins are blocked, followed by antibody incubation and detection using chemiluminescence, colorimetric, or fluorescent methods. Membrane immunodetection devices facilitate the processing of Western blots by simplifying workflows and accelerating detection times.

Related Product Resources

Protocol: Protein Blotting Handbook
Article: 30-minute Immunodetection Protocol Using the SNAP i.d.® 2.0 System
Brochure: Rethink Western Blotting

Snap i.d.® 2.0 system for autonomous immunodetection in Western blotting

SNAP i.d.® 2.0 Protein Detection System

The SNAP i.d.® 2.0 Protein Detection System is a unique, vacuum-driven system that pulls reagents through the membrane, increasing membrane contact while eliminating dependence on diffusion or agitation. This improvement in three-dimensional reagent distribution decreases the length of time required for immunodetection. While traditional Western blotting requires 4 to 24 hours for the blocking, antibody incubation, and washing steps, the SNAP i.d.® 2.0 protocol takes only 30 minutes with no loss of signal intensity or reduction in blot quality. All immunodetection steps after protein transfer to a membrane (i.e., blocking, washing, and primary and secondary antibody incubations) can be performed with the SNAP i.d.® 2.0 System.

Related Product Categories
Transfer Membranes & Kits
Choose from a variety of Immobilon® PVDF membranes for optimal Western blotting and protein immunoblotting performance based on target protein size, application, detection method, and convenience.
Transfer Buffers & Reagents
Premixed transfer buffers for consistent, reliable protein transfer and high purity, electrophoresis-grade chemicals for making transfer buffers for Western blotting applications.
Protein Gel Electrophoresis and Transfer Equipment
Electrophoresis tanks, blotting systems, and power supplies for protein gel electrophoresis and wet and semi-dry transfer.
Protein Electrophoresis Gels & Buffers
Precast polyacrylamide protein gels, running buffers, and hand-casting reagents for hand-pouring polyacrylamide gels for PAGE and SDS-PAGE protein gel electrophoresis.
Protein Electrophoresis Gel Stains
High sensitivity colorimetric and fluorescent protein gel stains, fixing solutions, and de-staining reagents for polyacrylamide gels, agarose gels, and PVDF and nitrocellulose membranes.
Protein Markers
Pre-stained and unstained molecular weight markers for protein gel electrophoresis, SDS-PAGE, and Western blotting applications.
Immunodetection Reagents & Substrates
High quality blocking reagents, secondary antibodies, and HRP and AP substrates for Western blotting and immunoblotting detection.
Related Applications
Western Blotting
An overview of the principles and applications of Western blotting for protein detection. Includes links to detailed protocols, videos, and other resources for protein extraction, gel electrophoresis, transfer to PVDF or nitrocellulose membranes, and chemiluminescent, colorimetric, and fluorescent detection methods.

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