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Astec® CHIROBIOTIC® V2 Chiral (5 μm) HPLC Columns

L × I.D. 25 cm × 4.6 mm, HPLC Column

Synonym(s):

Chirobiotic V2 TM=["Astec"] Chromatography Column

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

Astec® CHIROBIOTIC® V2 Chiral HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm

material

stainless steel column

Quality Level

100

agency

suitable for USP L88

description

HPLC column

product line

Astec®

packaging

pkg of 1 ea

manufacturer/tradename

Astec®

parameter

0-45 °C temperature
241 bar pressure (3500 psi)

technique(s)

HPLC: suitable
LC/MS: suitable

L × I.D.

25 cm × 4.6 mm

matrix

High-purity silica gel particle platform
fully porous particle

matrix active group

vancomycin phase

particle size

5 μm

pore size

200 Å

operating pH range

3.5-7.0

separation technique

chiral

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General description

Neutral molecules, amides, acids, esters and amines show considerable enantioselectivity on these vancomycin-based CSPs. A wide variety of secondary and tertiary amines have been separated on the CHIROBIOTIC® V in the polar ionic mode. The CHIROBIOTIC® V has demonstrated many of the separation characteristics of protein-based stationary phases, but with exceptional stability and much higher sample capacity. Some chiral analytes have been resolved that have not been reported separated on any other chiral stationary phase. CHIROBIOTIC® V and V2 differ in their bonding chemistry the pore size of the support particle, giving them different selectivity and preparative capacity.

  • Bonded phase: Vancomycin
  • Operating pH range: 3.5 - 7.0
  • Particle diameter: 5, 10 or 16 μm
  • Pore size: 100 Å (CHIROBIOTIC® V) or 200 Å (CHIROBIOTIC® V2)

CHIROBIOTIC FAQs
CHIROBIOTIC Reference Bibliography
Chiral Product Literature

Application


  • Chiral analysis of dextromethorphan and levomethorphan in human hair by liquid chromatography-tandem mass spectrometry: This study utilized the CHIROBIOTIC® V2 column to achieve chiral separation of dextromethorphan and levomethorphan in human hair, highlighting the column′s application in forensic toxicology for precise enantiomeric analysis (Ji et al., 2022).

  • Development and validation of an UFLC-MS/MS method for enantioselectivity determination of d,l-thero-methylphenidate, d,l-thero-ethylphenidate and d,l-thero-ritalinic acid in rat plasma: This research demonstrates the application of CHIROBIOTIC® V2 for pharmacokinetic studies in rats, establishing a method for enantioselective determination that can be crucial for drug development and therapeutic monitoring (Zhang et al., 2016).

  • New high-performance liquid chromatography method for the determination of (R)-warfarin and (S)-warfarin using chiral separation on a glycopeptide-based stationary phase: The use of CHIROBIOTIC® V2 column in developing a new HPLC method for the separation of warfarin enantiomers underscores its utility in clinical analysis and therapeutic drug monitoring, showing its broad applicability in biomedical research (Malakova et al., 2009).

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Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC

Application

Product No.
Description
Pricing

G004403

HPLC Analysis of Methadone Enantiomers on Astec® CHIROBIOTIC® V2, application for HPLC

G004457

HPLC Analysis of Acebutolol Enantiomers on Astec® CHIROBIOTIC® V2, application for HPLC

G004458

HPLC Analysis of 1-(1-Naphthyl)ethylamine Enantiomers on Astec® CHIROBIOTIC® V2, application for HPLC

G004463

HPLC Analysis of Amlodipine Enantiomers on Astec® CHIROBIOTIC® V2, application for HPLC

G004464

HPLC Analysis of Bupivacaine Enantiomers on Astec® CHIROBIOTIC® V2, Acetate Mobile Phase, application for HPLC

G004465

HPLC Analysis of Fluoxetine Enantiomers on Astec® CHIROBIOTIC® V2, application for HPLC

G004466

HPLC Analysis of Mepivacaine Enantiomers on Astec® CHIROBIOTIC® V2, application for HPLC

G004467

HPLC Analysis of Amphetamine and Methamphetamine Enantiomers on Astec® CHIROBIOTIC® V2, application for HPLC

G004468

HPLC Analysis of Dextromethorphan Enantiomers on Astec® CHIROBIOTIC® V2, application for HPLC

G004469

HPLC Analysis of Mianserin Enantiomers on Astec® CHIROBIOTIC® V2, application for HPLC

G004470

HPLC Analysis of Nicardipine Enantiomers on Astec® CHIROBIOTIC® V2, application for HPLC

G004473

HPLC Analysis of Thalidomide Enantiomers on Astec® CHIROBIOTIC® V2, application for HPLC

G004474

HPLC Analysis of Trimipramine Enantiomers on Astec® CHIROBIOTIC® V2, application for HPLC

G004475

HPLC Analysis of Warfarin Enantiomers on Astec® CHIROBIOTIC® V2, application for HPLC

G004477

HPLC Analysis of 5-Methyl-5-Phenylhydantoin Enantiomers on Astec® CHIROBIOTIC® V2, application for HPLC

G004478

HPLC Analysis of Dorzolamide Enantiomers on Astec® CHIROBIOTIC® V2, application for HPLC

G004504

HPLC Analysis of Methylphenidate (Ritalin) Enantiomers on Astec® CHIROBIOTIC® V2, application for HPLC

G004619

HPLC Analysis of Bupivacaine Enantiomers on Astec® CHIROBIOTIC® V2, Formate Mobile Phase, application for HPLC

G004655

HPLC Analysis of N-Desmethylnefopam Enantiomers on Astec® CHIROBIOTIC® V2, application for HPLC

G004656

HPLC Analysis of Oxamniquine Enantiomers on Astec® CHIROBIOTIC® V2, application for HPLC

G004657

HPLC Analysis of Tolperisone Enantiomers on Astec® CHIROBIOTIC® V2, application for HPLC

G004658

HPLC Analysis of Nebivolol Enantiomers on Astec® CHIROBIOTIC® V2, application for HPLC

G004659

HPLC Analysis of Methorphan Enantiomers on Astec® CHIROBIOTIC® V2, application for HPLC

G004660

HPLC Analysis of Meptazinol Enantiomers on Astec® CHIROBIOTIC® V2, application for HPLC

G004661

HPLC Analysis of Sibutramine Enantiomers on Astec® CHIROBIOTIC® V2, application for HPLC

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Certificates of Analysis (COA)

Lot/Batch Number
281513-04
281513-03
281513-02
281513-01
281502-04

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Chromatography Liquid Chiral Separations
Xiao, T.L., et al.
Encyclopedia of Separation Science, 1-15 (2000)
Enantioselective and sensitive determination of carvedilol in human plasma using chiral stationary-phase column and reverse-phase liquid chromatography with tandem mass spectrometry
Jiang, Juanjuan, et al.
Journal of Chromatography. B, Biomedical Sciences and Applications, 960, 92-97 (2014)
Enantioselective determination of doxazosin in human plasma by liquid chromatography?tandem mass spectrometry using ovomucoid chiral stationary phase
Liu, Ke, et al.
Journal of Chromatography. B, Biomedical Sciences and Applications, 878 (26), 2415-2420 (2010)
Quantitative determination of ondansetron in human plasma by enantioselective liquid chromatography-tandem mass spectrometry
Liu, Ke, et al.
Journal of Chromatography. B, Biomedical Sciences and Applications, 864 (1-2), 129-136 (2008)
Validation of a chiral liquid chromatography?tandem mass spectrometry method for the determination of pantoprazole in dog plasma
Chen, Meixia, et al.
Journal of Chromatography. B, Biomedical Sciences and Applications, 906, 85-90 (2012)
View All Related Papers

Protocols

Chiral LC-MS Analysis of Methamphetamine in Urine on Astec® CHIROBIOTIC® V2

Efficient LC-MS separation of methamphetamine enantiomers in urine allows sensitive differentiation of illicit D-methamphetamine from over-the-counter L-methamphetamine.

Accurate Biological Testing for Amphetamine and Methamphetamine Abuse Using Chiral HPLC and MS Detection

Optimized sample prep and chiral chromatography methods for the LC/MS analysis of these drug enantiomers in urine

Biological Testing for Amphetamine and Methamphetamine Abuse

In this study, optimized methods are presented for sample preparation and chiral chromatography for the LC/MS analysis of amphetamine and methamphetamine enantiomers in urine.

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