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G004403

HPLC Analysis of Methadone Enantiomers on Astec® CHIROBIOTIC® V2

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: CHIROBIOTIC V2, 25 cm x 4.6 mm I.D., 5 μm particles (15024AST)
mobile phase: [A] methanol; [B] 20 mM ammonium formate; (95:5, A:B)
flow rate: 1 mL/min
column temp.: 20 °C
detector: UV, 205 nm
injection: 2 μL
sample: methadone, 1 mg/mL in mobile phase

suitability

application for HPLC

application(s)

clinical
forensics and toxicology

Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC

Analyte

Description
    (±)-Methadone hydrochloride, powder, ≥98%

analytical column

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Description
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standard

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