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RK20407

Sigma-Aldrich

ABScript II One Step RT-qPCR Probe Kit

Synonym(s):

One Step RT-qPCR master mix, Probe-based RT-qPCR

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About This Item

usage

sufficient for 100 reactions
 sufficient for 20 reactions
 sufficient for 500 reactions

feature

dNTPs included
hotstart

technique(s)

DNA amplification: suitable
PCR: suitable
RT-PCR: suitable
RT-qPCR: suitable
cDNA synthesis: suitable
qPCR: suitable

input

purified RNA

compatibility

ABI 7000
 ABI 7300
 ABI 7500
 ABI 7700
 ABI 7900
 ABI StepOne
 ABI StepOnePlus
 ABI ViiA 7
 Bio-Rad CFX384
 Bio-Rad CFX96
 Bio-Rad iCycler
 Eppendorf® Mastercycler ep realplex2 s
 Eppendorf® Mastercycler ep realplex
 Qiagen Corbett Rotor-Gene 3000
 Qiagen Corbett Rotor-Gene 6000
 Qiagen Corbett Rotor-Gene Q
 QuantStudio™
 Roche LightCycler 480
 Strategene Mx3000P
 Strategene Mx3005P
 Strategene Mx4000

shipped in

dry ice

storage temp.

−20°C

General description

The ABScript II One Step RT-qPCR Probe Kit is a convenient, ready-to-use kit with optimized components for use for reverse transcription and subsequent probe-based qPCR. The one-step format prevents contamination and allows for higher sensitivity. This kit is ideal for analyzing low input RNA samples.The ABScript II Reverse Transcriptase in the kit provides reliable reverse transcription of RNA. After reverse transcription, the hot-start version of Taq polymerase is activated at 95°C and the ABScript II Reverse Transcriptase is inactivated simultaneously. In the sequential PCR reaction, the 5′-3′ exonuclease activity of Taq polymerase cleaves the hybridized probe, separating the reporter from the quencher and releasing fluorescent signal.

Application

ABScript II One Step RT-qPCR Probe Kit is designed for use in real-time RT-PCR (or RT-qPCR) applications, including detection of RNA in samples being analyzed.

For Research Use Only.

Application Note

Additional Materials Required but Not Included:

  • RNA templates, primers and probes
  • Optical-grade qPCR tubes, plates, sealing films, and aerosol-resistant pipette tips

Compatible Instruments:

*50X ROX Reference Dye I
Applied Biosystems 7000/7300/7700/7900, Applied Biosystems StepOne™/StepOnePlus™

**50X ROX Reference Dye II
Applied Biosystems 7500/ViiA™7, QuantStudio, Stratagene Real-time PCR Systems, Rotor-gene® 3000

NO ROX Reference Dye
Bio-Rad iCyclers/ CFX96™/ CFX384™, Roche® Light Cyclers, QIAGEN/Corbett Systems, Eppendorf Mastercyclers®.

Features and Benefits

  • Ready-to-use one-step RT-qPCR probe kit with all components for RT-qPCR (except primers, probes and template RNA)
  • Uses the robust ABScript II Reverse Transcriptase (RT), which provides reliable and efficient reverse transcription on a wide range of RNA template amount
  • This one-step kit improves sensitivity and effectively prevents contamination
  • Flexible format, as the ABScript II One Step RT-qPCR Probe Kit includes two separate 50X concentrations of the ROX (passive reference) dye, to be added as required by specific qPCR instruments.

Components

2X One Step RT-qPCR Probe Buffer*
One Step Probe HS Taq
ABScript II RT Enzyme Mix
50X ROX Dye I *
50X ROX Dye II **
RNase-free ddH2O
See datasheet for more information.

Reaction Size
Reaction size is based on a 50 uL reaction volume.

The 2x One Step RT-qPCR Probe Buffer is supplied in the following volumes:

20RXN: 0.5 mL

100RXN: 2.5 mL

500RXN: 1.25 mL

Legal Information

CFX384 is a trademark of Bio-Rad Laboratories, Inc.
CFX96 is a trademark of Bio-Rad Laboratories, Inc.
Eppendorf is a registered trademark of Eppendorf AG
Mastercycler is a registered trademark of Eppendorf AG
QuantStudio is a trademark of IROA Technologies LLC
Roche is a registered trademark of Roche Molecular Systems, Inc.
Rotor-Gene is a registered trademark of Qiagen GmbH
StepOne is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
StepOnePlus is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
ViiA is a trademark of ThermoFisher

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Related Content

RT-qPCR – Quantitative Reverse Transcription PCR

RT-qPCR, or quantitative reverse transcription PCR, combines the effects of reverse transcription and quantitative PCR or real-time PCR to amplify and detect specific targets. RT-qPCR has a variety of applications including quantifying gene expression levels, validating RNA interference (RNAi), and detecting pathogens such as viruses.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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