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I7403

Sigma-Aldrich

L-Isoleucine

98.5-101.0%, suitable for cell culture, BioXtra, non-animal source

Synonym(s):

(2S,3S)-2-Amino-3-methylpentanoic acid

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About This Item

Linear Formula:
C2H5CH(CH3)CH(NH2)CO2H
CAS Number:
73-32-5
Molecular Weight:
131.17
Beilstein/REAXYS Number:
1721792
EC Number:
200-798-2
MDL number:
MFCD00064222
UNSPSC Code:
12352209
eCl@ss:
32160406
PubChem Substance ID:
24896115
NACRES:
NA.26

product name

L-Isoleucine, from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, 98.5-101.0%

biological source

non-animal source

agency

USP/NF
meets EP testing specifications
meets JP testing specifications
meets USP testing specifications

product line

BioXtra

assay

98.5-101.0%

form

powder

quality

meets EP, JP, USP testing specifications

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

color

white

mp

288 °C (dec.) (lit.)

solubility

1 M HCl: 50 mg/mL

application(s)

peptide synthesis
pharmaceutical (small molecule)

SMILES string

CC[C@H](C)[C@H](N)C(O)=O

InChI

1S/C6H13NO2/c1-3-4(2)5(7)6(8)9/h4-5H,3,7H2,1-2H3,(H,8,9)/t4-,5-/m0/s1

InChI key

AGPKZVBTJJNPAG-WHFBIAKZSA-N

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Application

L-Isoleucine has been used as a supplement of maintenance media consisting of DMEM/F-12 for the culture of primary hepatocytes. It has also been used as a supplement for serial culture of adult murine keratinocytes.

Biochem/physiol Actions

L-Isoleucine is an essential amino acid that is synthesized from threonine. It is a branched-chain hydrophobic amino acid. L-isoleucine is an isomer of L-leucine.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

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Conversion of ammonia or urea into essential amino acids,L-leucine,L-valine, andL-isoleucine using artificial cells containing an immobilized multienzyme system and dextran-NAD+
Kang Fu Gu
Applied Biochemistry and Biotechnology, 26 (1990)
Biochemistry (5th Edition) null
Inhibitory effect of aroma on the bitterness of branched-chain amino acid solutions.
Mukai J
Chemical & Pharmaceutical Bulletin, 55(11), 1581-1584 (2007)
Serial Cultivation of Primary Adult Murine Keratinocytes
Richard P. Redvers and Pritinder Kaur
Methods in Molecular Biology (2005)
Recapitulation of metabolic defects in a model of propionic acidemia using patient-derived primary hepatocytes.
Chapman KA
Molecular Genetics and Metabolism, 117(3), 355-362 (2016)
View All Related Papers

Chromatograms

application for HPLC

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