T9060
BlueView™ Nucleic Acid Stain
in 10× TBE buffer, powder blend
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About This Item
MDL number:
UNSPSC Code:
12161700
grade
for molecular biology
form
powder blend
detection sensitivity measuring range
<250 ng/band
storage temp.
room temp
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Application
BlueView is a quick, safe alternative to ethidium bromide staining for nucleic acid electrophoresis. BlueView can be used directly as the running buffer and in the gel for instant staining of the bands of nucleic acids during electrophoresis, visible in ambient light. Bands can be excised from the gel without the use of UV which can cause nicking. BlueView stained bands can be used for PCR, ligation, labeling, restriction digestion, and Southern blotting.
Reconstitution
Reconstitution with water produces a 10× stock solution of the BlueView stain in 10× TAE or TBE buffer. The bottles are large enough to contain the concentrate. Dilute the 10× stock solution to the desired concentration before use. Concentrations of TBE and TAE between 0.5 and 1× are commonly used for nucleic acid electrophoresis. A 20-fold dilution of the stock solution is recommended for low background staining and optimum signal to noise.
Legal Information
BlueView is a trademark of Sigma-Aldrich Co. LLC
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Repr. 1B
Storage Class Code
6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 1
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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