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PURE1A

Sigma-Aldrich

Protein A Antibody Purification Kit

sufficient for 10 purifications

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.32

usage

sufficient for 10 purifications

shipped in

wet ice

storage temp.

2-8°C

General description

The Protein A Antibody Purification Kit provides all of the reagents necessary to isolate monoclonal and polyclonal antibodies.

Specificity

Capacity/Run (by Species)
Human IgG 20-25 mg
Mouse IgG1 8-12 mg
Mouse IgG2a 10-15 mg
Mouse IgG2b 6-10 mg
Mouse IgG3 15-20 mg
Rabbit IgG 8-12 mg
Goat IgG 2-4 mg
Bovine IgG 8-12 mg

Application

PURE-1A offers Protein A technology in a prepackaged, easy to use kit form. With this kit, milligram quantities of IgG can be purified from serum, ascites, or cell culture supernatants.
Purification of IgG from other species may be possible, however, the researcher will have to determine the suitability of the kit for their application.
The Protein A Antibody Purification Kit is suitable for use with IgGs from human, mouse, rabbit, goat, and bovine species.

Features and Benefits

  • High capacity - purify up to 8 mg of mouse IgG or 25 mg of human IgG per column run
  • Specific - will only bind IgG
  • Easy to use - antibody is eluted and desalted in a single step, ready to use
  • Gentle - avoids prolonged exposure of the antibody to low pH

Principle

Using our cartridge system, antibodies elute as highly purified proteins at physiological pH. Protein A is a powerful tool for isolation of antibodies from mammalian hosts. Protein A exhibits a high degree of specificity for IgG and ensures an antibody preparation virtually free of IgA, IgM and non-immunoglobulin serum proteins such as albumin.

Kit Components Only

Product No.
Description
  • Binding Buffer 225 mL
  • Desalting Cartridge 1 ea
  • Elution Buffer 75 mL
  • HEPES Buffer 225 mL
  • 5 ml Syringe 1
  • 10 ml Syringe 1
  • Protein A Cartridge 1 mL
  • Regeneration Buffer 75 mL
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Related product

Product No.
Description
Pricing

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

hcodes

H302,H370

Hazard Classifications

Acute Tox. 4 Oral - STOT SE 1

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

Antibody Purification using Protein A, Protein G, or Protein L Agarose

Antibody Purification using Protein A, Protein G, or Protein L Agarose protocol is designed as a quick purification method for antibodies from mammalian sera, ascites, and cell culture supernatants. It should be noted that if the starting material is serum or ascites the final preparation will contain endogenous host IgG as well as specific antibodies. In general, the presence of this endogenous IgG should not interfere with assays using the antibodies.

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