MilliporeSigma
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IP05

Millipore

Protein G Plus/Protein A Agarose Suspension

Protein G PLUS/Protein A-Agarose mixture specifically formulated for immunoprecipitation.

form

slurry (Liquid)

contains

≤0.1% sodium azide as preservative

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

shipped in

wet ice

storage temp.

2-8°C

General description

Designed for immunoprecipitation applications. This product is blocked with BSA to reduce non-specific binding and cannot be used for purification.
Protein G PLUS/Protein A-Agarose mixture specifically formulated for immunoprecipitation.

Packaging

1.5 ml in Plastic ampoule

Application

Immunoprecipitation (see comments)

Warning

Toxicity: Standard Handling (A)

Physical form

33% slurry in PBS.

Other Notes

Agarose solution is supplied ready to use. Protein G Plus/Protein A Agarose immunoprecipitation reagent is blocked with BSA and should not be used for immunoglobulin purification or covalent cross-linking. For immunoprecipitation reactions 15 µl of solution per µg primary antibody is recommended. Preclearing will minimize extra bands resulting from nonspecific precipitation. To preclear, add to the sample 20 µl of agarose conjugate and 1 µg of normal IgG from the same species as the immunoprecipitating antibody. When immunoblotting is used for detection, some secondary antibodies can react nonspecifically with BSA or other proteins present at high concentrations in the sample. This can be eliminated by reducing the concentration of secondary antibody.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Quality

Enter Lot Number to search for Certificate of Quality (COQ).

Zhi-Gang Mei et al.
Aging, 12(13), 13187-13205 (2020-07-06)
Cerebral ischemia/reperfusion (CIR) injury occurs when blood flow is restored in the brain, causing secondary damage to the ischemic tissues. Previous studies have shown that electroacupuncture (EA) treatment contributes to brain protection against CIR injury through modulating autophagy. Studies indicated

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