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P9279

Sigma-Aldrich

Phospholipase A2 from honey bee venom (Apis mellifera)

salt-free, lyophilized powder, 600-2400 units/mg protein

Synonym(s):

Lecithinase A, PLA2, Phosphatidylcholine 2-acylhydrolase

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About This Item

CAS Number:
9001-84-7
Enzyme Commission number:
3.1.1.4 (BRENDA, IUBMB)
EC Number:
232-637-7
MDL number:
MFCD00131890
UNSPSC Code:
12352204
NACRES:
NA.32

form

salt-free, lyophilized powder

Quality Level

200

specific activity

600-2400 units/mg protein

mol wt

14.5 kDa

UniProt accession no.

P00630

storage temp.

−20°C

Gene Information

honey bee ... Pla2(406141)

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General description

Phospholipase A2 from honey bee venom (Apis mellifera) is a calcium-dependent lipolytic enzyme.

Application

Phospholipase A2 from honey bee venom (Apis mellifera) has been used
  • to determine renal proximal tubular segments (PTS) viability during oxygenation and hypoxia-reoxygenation
  • in the treatment of photosystem-II (PSII) dimer complex of Synechocystis to study changes in oxygen-evolving activity
  • as a standard in phospholipase activity assay

Biochem/physiol Actions

Hydrolyzes the β-ester bond of zwitterionic glycerophospholipids. Preferred substrates are phosphatidylcholine, phosphatidylethanolamine, and their plasmalogen analogues. Phosphatidylinositol and phosphatidylserine are also hydrolyzed. Aggressively attacks phospholipids in membranes of intact cells.

Unit Definition

One unit will hydrolyze 1.0 μmole of soybean L-α-phosphatidylcholine to L-α-lysophosphatidylcholine and a fatty acid per min at pH 8.9 at 25 °C.

Analysis Note

Protein determined by biuret.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Milleporin-1, a new phospholipase A2 active protein from the fire coral Millepora platyphylla nematocysts.
Radwan FF and Aboul-Dahab HM
Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP, 139(4), 267-272 (2004)
Lipids in oxygen-evolving photosystem II complexes of cyanobacteria and higher plants.
Sakurai I
Journal of Biochemistry, 140(2), 201-209 (2006)
Third calcium ion found in an inhibitor-bound phospholipase A2.
Sekar K
Acta Crystallographica Section D, Biological Crystallography, 62, 392-397 (2006)
Phospholipase A2 activity can protect renal tubules from oxygen deprivation injury.
Zager RA
Proceedings of the National Academy of Sciences of the USA, 90(17), 8297-8301 (1993)
Ming Chen et al.
Scientific reports, 7(1), 15931-15931 (2017-11-23)
Hepatitis C virus (HCV), dengue virus (DENV) and Japanese encephalitis virus (JEV) belong to the family Flaviviridae. Their viral particles have the envelope composed of viral proteins and a lipid bilayer acquired from budding through the endoplasmic reticulum (ER). The
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