MilliporeSigma
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P8913

Sigma-Aldrich

Phospholipase A2 from bovine pancreas

lyophilized powder, ≥20 units/mg protein

Synonym(s):
Lecithinase A, PLA2, Phosphatidylcholine 2-acylhydrolase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.32

form

lyophilized powder

Quality Level

specific activity

≥20 units/mg protein

composition

Protein, 5-20% biuret

UniProt accession no.

storage temp.

−20°C

Gene Information

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General description

Phospholipase A2 from bovine pancreas is a calcium-dependent lipolytic enzyme.[63]

Application

Phospholipase A2 from bovine pancreas has been used to determine rat renal proximal tubular segments (PTS) viability during oxygenation and hypoxia-reoxygenation[98] and to study the impact of phospholipase A2 on tubular cell triglyceride levels.[22][24]

Packaging

5 mg in glass bottle
10, 50 mg in poly bottle
Package size based on protein content

Biochem/physiol Actions

Hydrolyzes the β-ester bond of zwitterionic glycerophospholipids. Preferred substrates are phosphatidylcholine, phosphatidylethanolamine, and their plasmalogen analogues. Phosphatidylinositol and phosphatidylserine are also hydrolyzed. Aggressively attacks phospholipids in membranes of intact cells.

Unit Definition

One unit will hydrolyze 1.0 μmole of soybean L-α-phosphatidylcholine to L-α-lysophosphatidylcholine and a fatty acid per min at pH 8.0 and 37 °C.

Physical form

Contains Tris buffer salts.

Analysis Note

Protein determined by biuret.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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