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Key Documents

63301-011

Sigma-Aldrich

PCRboost®

1 mL bottle, for improved PCR performance

Synonym(s):

PCRboost®

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.52

form

liquid

usage

28 reactions

storage temp.

room temp

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General description

PCRboost® is a unique reagent that enhances end-point and reverse transcription-PCR performance by improving sensitivity and specificity during amplification of genomic DNA or RNA templates. To use, simply replace the water in your PCR with PCRboost®. That′s all. It is designed to enhance the amplification of DNA without the need for time consuming optimizations. PCRboost® successfully amplifies thermally degraded DNA resulting in a visible amplicon that can be used for downstream applications such as cloning and sequencing.

Don′t see what you are looking for? Custom stabilization services for molecular diagnostic assay and reagent manufacturers are available through Sigma′s partner, Biomatrica. PCRstable® enables the preservation of PCR-based assay components in any format (liquid or dry).

Features and Benefits

Boost PCR results for:

  • Degraded FFPE samples and bisulphite-treated samples (methylation studies)
  • Multiplex PCR
  • Low-copy transcripts
  • Samples containing inhibitory factors (i.e. humic acid, heme, and indigo dye)

No changes to your PCR protocol. Simply replace the water in 
your reaction.

PCR products are compatible with all downstream applications.

Packaging

1 bottle

Physical form

1.0 mL PCRboost® in a 1.5 mL screw-top conical tube

Legal Information

PCRboost is a registered trademark of Biomatrica
PCRstable is a registered trademark of Biomatrica

Related product

Product No.
Description
Pricing

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

Successful Amplification of Thermally Degraded DNA Using PCRboost™

DNA samples are often exposed to harsh environmental conditions, including heat, humidity, UV or chemicals. Some procedures, such as the extraction of samples from formalin-fixed paraffin embedded tissue samples, subject DNA to multiple extreme conditions.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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