EDEGLY
Enzymatic Protein Deglycosylation Kit
Synonym(s):
Glycosylation Release Kit
About This Item
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General description
Application
- protein deglycosylation
- deglycosylation of tissue factor pathway inhibitor (TFPI) α and β
- deglycosylation of native exo-β-(1,3)-glucanase EXG1
Features and Benefits
- two or more mg of glycoprotein is deglycosylated
- single reaction at neutral pH
- native & denaturing procedures
- no degradation of protein
- O-linked sugars containing polysialic acid are eliminated
- control glycoprotein provided
Kit Components Only
- PNGase F 1 mL/vial
- O-Glycosidase 20 μL
- α-2(3,6,8,9)-Neuraminidase 20 μL
- Fetuin Control .5 mg
- 5× Reaction Buffer .2 mL
- Denaturation Solution .1 mL
- Triton™ X-100 .1 mL
- β-1→4-Galactosidase, positionally specific 20 μL
- β-N-Acetylglucosaminidase 20 μL
Related product
signalword
Danger
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Articles
Information about Glycoprotein deglycosylation. The diversity of oligosaccharide structures, both O-linked and N-linked, often results in heterogeneity in the mass and charge of glycoproteins.
There is no enzyme comparable to PNGase F for removing intact O-linked sugars. Monosaccharides must be sequentially hydrolyzed by a series of exoglycosidases until only the Gal-b(1-3)-GalNAc core remains. O-Glycosidase can then remove the core structure intact with no modification of the serine or threonine residue.
Deglycosylation Kits
Post-translational modifications such as glycosylation, phosphorylation, and sulfation, to name a few, serve many functions. As a result, the analysis of proteins and their post-translational modifications is particularly important for the study of diseases where multiple genes are known to be involved, such as heart disease, cancer and diabetes.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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