MilliporeSigma
All Photos(1)

EDEGLY

Sigma-Aldrich

Enzymatic Protein Deglycosylation Kit

All Photos(1)
Synonym(s):
Glycosylation Release Kit
NACRES:
NA.32

Quality Level

200

shipped in

wet ice

storage temp.

2-8°C

General description

Enzymatic protein deglycosylation kit contains all the enzymes and reagents required for the complete removal of N-linked and simple O-linked carbohydrates from glycoproteins. This kit is employed to cleave the complex Core 2 O-linked carbohydrates including those containing polylactosamine.

Application

Enzymatic protein deglycosylation kit has been used in:
  • protein deglycosylation
  • deglycosylation of tissue factor pathway inhibitor (TFPI) α and β
  • deglycosylation of native exo-β-(1,3)-glucanase EXG1

Enzymatic Protein Deglycosylation Kit has been used for the deglycosylation of various enzymes.

Features and Benefits

Enzymatic protein deglycosylation kit has the following features:
  • two or more mg of glycoprotein is deglycosylated
  • single reaction at neutral pH
  • native & denaturing procedures
  • no degradation of protein
  • O-linked sugars containing polysialic acid are eliminated
  • control glycoprotein provided

Kit Components Only

Product No.
Description
  • PNGase F 1 mL/vial
  • O-Glycosidase 20 μL
  • α-2(3,6,8,9)-Neuraminidase 20 μL
  • Fetuin Control .5 mg
  • 5× Reaction Buffer .2 mL
  • Denaturation Solution .1 mL
  • Triton™ X-100 .1 mL
  • β-1→4-Galactosidase, positionally specific 20 μL
  • β-N-Acetylglucosaminidase 20 μL
See All (9)

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Product Information Sheet

Product Information Sheet - EDEGLY

More Documents

GlycoProfile I Enzymatic In-gel N-Deglycosylation Kit

Quotes and Ordering

Bulk Quote-Order Product

Articles

Glycoprotein Deglycosylation

Information about Glycoprotein deglycosylation. The diversity of oligosaccharide structures, both O-linked and N-linked, often results in heterogeneity in the mass and charge of glycoproteins.

O-Linked Glycan Strategies

There is no enzyme comparable to PNGase F for removing intact O-linked sugars. Monosaccharides must be sequentially hydrolyzed by a series of exoglycosidases until only the Gal-b(1-3)-GalNAc core remains. O-Glycosidase can then remove the core structure intact with no modification of the serine or threonine residue.

Deglycosylation Kits

Deglycosylation Kits

Post Translational Modification

Post-translational modifications such as glycosylation, phosphorylation, and sulfation, to name a few, serve many functions. As a result, the analysis of proteins and their post-translational modifications is particularly important for the study of diseases where multiple genes are known to be involved, such as heart disease, cancer and diabetes.

See All

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service