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MilliporeSigma

Cultivate Consistency

Thousands of tools and reagents for reproducible cell culture

Cell culture is fundamental to life science research in universities, biotechnology, pharmaceuticals, and industry. Whether your goal is to establish relevant cell models to answer research questions or to produce recombinant proteins or virus particles, we have everything you need to keep your tissue culture room thriving. Our complete portfolio offers plenty of choices for quality essentials from fetal bovine serum (FBS), culture media, supplements, and cryopreservation reagents to filtration units, general labware, and specialty cultureware. Through partnerships with organizations like the European Collection of Authenticated Cell Cultures (ECACC), we remain committed to providing authenticated, validated, and frequently-cited tools for the culture of mammalian cells. For plant tissue culture techniques and solutions, click here.

Cell Culture Workflow

Explore cell lines and specialty cell culture.

Cell Lines & Specialty Cell Culture

We’ve partnered with the European Collection of Authenticated Cell Cultures (ECACC) repository to offer quality cell lines.  Browse our portfolio of authenticated, validated, mycoplasma-free cell lines for biomedical research, protein production, hybridoma fusion, virus propagation, and more — including hundreds of cancer cell lines, and iPS cells from the European Bank of induced Pluripotent Stem Cells (EBiSC).

Together with ECACC, we offer a carefully curated,  diverse selection of cell lines and expert culture protocols. Our primary cells include premier human lines, media, and protocols. Browse a wide range of tools for innovative approaches to stem cell work..

Browse our wide selection of FBS and sera from other species.

Along with our rigorously-tested, fully-documented and reliable supply of FBS and other sera, our tested cell culture media formulations, buffers and salts provide the appropriate environment for healthy propagation of any cell phenotype. 

Fit-for-phenotype, high performance cell culture media and sera ensure maximum yield for consistent models and reproducible results. Choose from powdered or liquid formats, plus defined media and serum replacements for systems requiring serum-free conditions.

Find a complete range of supplements for cell culture media.

From trypsin to antibiotics, growth factors, cytokines and more, our complete range of culture-tested supplements and reagents promote cell health while protecting cultures from contamination and stress. 

Our cell culture-tested reagents undergo validation in relevant culture systems, to eliminate the need for screening biochemicals prior to use in your cell culture application. Find growth factors and dissociation reagents for every culture need. 

Explore organoids, hydrogels, bioinks, and more innovative 3D cell culture tools.

Our human organoids and organoid culture systems are the latest innovations in our portfolio of leading-edge 3D cell culture products that includes scaffolds and hydrogels. Human skin model systems and bioinks for 3D printing expand your options for creating more biorelevant in vitro models.

3D cell culture systems enable models that recapitulate in vivo environments. Choose from scaffold-based or scaffold-free systems enabling culture of spheroids and organoids derived from stem cells, patient samples, and more.

Related Applications
Cell Culture Troubleshooting
Expert tips for correcting common problems with cell culture including bacterial and fungal contamination, debris in culture media, cell detachment or clumping, slow growth, and low viability.
Cell Culture Media Preparation
Culture media preparation for in vitro cell cultivation including preparation of different media formats, sterilization methods, cell growth and regulation supplements, media storage and use.
Cell Growth & Maintenance
A synopsis of the conditions, media, nutrients, cultureware, and environments required for successful propagation of mammalian and yeast cells. Nutrient reagents, sterile technique and filtration, and requirements for 2D, 3D, and suspension cultures are described.
3D Cell Culture
Applications, principles, and techniques for three-dimensional (3D) cell culture. Includes techniques and descriptions of scaffold and scaffold-free methods matrices for the culture of complex in vitro models including organoids and spheroids.
Cell Analysis
An overview of cellular analysis methods including detailed protocols, technical notes, and videos covering cell counting and cytometry, cell imaging techniques, and cell-based assays.
Cell Culture by Cell Type
Methods for plant, microbial, and mammalian tissue culture including growth and maintenance of immortalized cell lines, primary cells, stem cells, and 3D cell cultures for basic research, agricultural development, drug discovery and manufacturing, and clinical research.
Transfection & Gene Editing
Cell transfection principles, common transfection methods, and the role of transfection in gene editing and silencing for research, drug discovery, recombinant protein production.
Cell Counting & Health Analysis
Techniques for counting cells and monitoring cell health and viability in culture, including calorimetric and fluorescent cell viability and proliferation assays. Methods for manual or automated cell counting include Coulter devices.
Related Services
Cell Line Development
Our full cell line development capabilities and expertise span from DNA and vector constructs to top clonal selection, research and master cell banks, and stability studies.
Related Product Categories
Cell Culture Media & Buffers
Standard, complex, and specialty cell culture media for a variety of cell lines and applications. We also offer starting buffers and supplements used in custom media development.
Cell Counting Equipment
Ensure precise cell counts: handheld coulter counter delivers precise, rapid results. Opt for disposable hemocytometers in infectious environments.
Cell Health Analysis
Protect valuable cell cultures and assess the effects of experimental treatments using assays for cell viability, proliferation, toxicity, and detection/elimination of mycoplasma and other culture contaminants.
CellASIC® ONIX2 Microfluidics
The CellASIC® ONIX2 Microfluidic System is a powerful, automated platform that allows precise manipulation of the cell culture environment for live-cell imaging and analysis using mammalian, yeast, and bacterial cells.
Cell Culture Supplements & Reagents
Browse our selection of high-quality cell culture supplements and reagents, including sera, attachment factors, growth factors, cytokines, hormones, cell dissociation reagents, and cell freezing reagents.
Cell Lines & Specialty Cell Culture
Browse our vast selection of mammalian, iPS, iPSC, and stem cell lines, including those from ECACC, PromoCell, and CAI.
Sterile Lab Media Filtration & Cultureware
Browse our selection of sterile laboratory filtration and cultureware products, including Stericup® vacuum driven filter units and Multiscreen filter plates.
Biochemicals
We offer a large selection of competatively priced, high-quality biochemicals, including buffers, detergents, agarose, lipids, antibiotics, metabolites, vitamins, and coenzymes.
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