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Key Documents

GE29-0181-84

HiPrep Phenyl High Performance16/10

Cytiva 29-0181-84, pack of 1 column

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About This Item

UNSPSC Code:
13111000
NACRES:
NA.56

ligand

phenyl

packaging

pack of 1 column

manufacturer/tradename

Cytiva 29-0181-84

parameter

1-5 ml/min flow rate
44 psi

bed size

16 mm × 100 mm

column I.D.

16 mm

matrix

6% cross-linked agarose

particle size

24-44 μm

avg. part. size

34 μm

cleaning

2-14

working range

3-13

suitability

suitable for bioprocess medium (BioProcess medium.)
suitable for bioprocess medium

General description

HiPrep 16/10 Phenyl HP is a prepacked column designed for preparative hydrophobic interaction chromatography (HIC) with good resolution. Phenyl Sepharose HP chromatography resin is based on a matrix of highly cross-linked agarose beads, which have been derivatized with phenyl groups. These are attached to the agarose matrix via uncharged, chemically stable ether linkages. The result is a truly hydrophobic resin with no ionic properties. High mechanical strength of the matrix allows for high flow rates, together with good resolution. Capacities vary according to the protein applied and its concentration. Loading capacity will also vary depending on the salt concentration, pH, and temperature at which the protein is applied.

Features and Benefits

  • Aromatic HIC resin for intermediate purification and polishing.
  • Selectivity similar to Phenyl Sepharose 6 Fast Flow (Low Sub) but higher resolution due to the smaller 34 µm bead size.
  • Provides fast, easy high-capacity separations in a convenient format.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

HiPrep is a trademark of Cytiva

Related product

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Warning

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Storage Class

3 - Flammable liquids


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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