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GE17-1082-01

Phenyl Sepharose™ High Performance

Cytiva 17-1082-01, pack of 75 mL

Sinónimos:

Phenyl sepharose

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

ligand

phenyl

packaging

pack of 75 mL

manufacturer/tradename

Cytiva 17-1082-01

matrix

6% cross-linked agarose

particle size

24-44 μm

avg. part. size

34 μm

cleaning in place

2-14

working range

3-13

suitability

suitable for bioprocess medium (BioProcess medium.)
suitable for bioprocess medium

General description

Phenyl Sepharose™ High Performance is an aromatic hydrophobic interaction chromatography (HIC) medium, designed for intermediate and polishing step purification steps when high resolution has priority.

Features and Benefits

  • Aromatic HIC medium designed for intermediate purification and polishing steps
  • Selectivity similar to Phenyl Sepharose™ 6 Fast Flow (low sub) but higher resolution due to the smaller 34 μm bead size
  • BioProcess medium supported for industrial applications and well-established in approved processes
  • The hydrophilic nature of the base matrix ensures low levels of non-specific binding

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Storage Class

3 - Flammable liquids

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Certificados de análisis (COA)

Lot/Batch Number

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Artículos

Salt Selection and Buffer Preparation

The correct choice of salt and salt concentration are the most important parameters that influence capacity and final selectivity. The objective is to optimize conditions to achieve the required selectivity to bind the target protein(s) and ensure that the majority of impurities pass through the column.

Column Packing and Preparation for Hydrophobic Interaction and Reversed Phased Chromatography

This page discusses column packing and preparation techniques for reverse phase chromatography.

HIC as a Capture Step

This page shows Hydrophobic Interaction Chromatography (HIC) in a purification strategy.

Column, Media and Sample Preparation for Hydrophobic Interaction Chomatography

Here the correct ways for Column, Media and Sample preparation for Hydrophobic Interaction Chomatography (HIC) with Cytiva media are detailed, including sample application, load volume and temperature

Protocolos

Screening for Selectivity of HIC Medium

This section is about the different ways of screening for selectivity of HIC medium from Cytiva, considering both automated and manual

Selectivity and the Properties of a HIC Medium

Selectivity of HIC medium from Cytiva is based on properties such as hydrophobicity, porosity and particle size.

Sepharose® High Performance: Purification with High Resolution

Here we discuss purification options when working with Sepharose® High Performance and we present some examples of media screening, capture and purification.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico