GE17-5195-01
HiTrap® Phenyl High Performance
Cytiva 17-5195-01, pack of 5 × 5 mL
Sinónimos:
HiTrap Phenyl Column, Phenyl High Performance
About This Item
Productos recomendados
ligand
phenyl
packaging
pack of 5 × 5 mL
manufacturer/tradename
Cytiva 17-5195-01
parameter
42 psi
bed size
16 mm × 25 mm
bed volume
5 mL
column I.D.
16 mm
matrix
6% cross-linked agarose
particle size
24-44 μm
avg. part. size
34 μm
cleaning
2-14
working range
3-13
suitability
suitable for bioprocess medium (BioProcess medium.)
suitable for bioprocess medium
General description
Features and Benefits
- Provide convenient and fast small-scale protein purifications.
- Phenyl Sepharose™ High Performance is a well-established aromatic HIC medium, designed for intermediate and polishing steps where high resolution has priority.
- Flexible design for use with syringes, single laboratory pump, or chromatography system such as AKTA design.
- High flow rates and high capacities.
- Excellent and easy scale-up achieved by connecting several HiTrap® columns in series or using HiPrep™ columns.
Storage and Stability
Analysis Note
Other Notes
Legal Information
Related product
signalword
Warning
hcodes
Storage Class
3 - Flammable liquids
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Artículos
The correct choice of salt and salt concentration are the most important parameters that influence capacity and final selectivity. The objective is to optimize conditions to achieve the required selectivity to bind the target protein(s) and ensure that the majority of impurities pass through the column.
This page discusses column packing and preparation techniques for reverse phase chromatography.
Here the correct ways for Column, Media and Sample preparation for Hydrophobic Interaction Chomatography (HIC) with Cytiva media are detailed, including sample application, load volume and temperature
Protocolos
This section is about the different ways of screening for selectivity of HIC medium from Cytiva, considering both automated and manual
Here we discuss purification options when working with Sepharose® High Performance and we present some examples of media screening, capture and purification.
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