The combination of Duolink® PLA technology and flow cytometry allows quantitative detection of protein-protein interactions, post-translational modifications, and protein expression with greater statistical power. In order to get the best results, a lot of planning, prep work, and troubleshooting is required. This guide provides general tips and tricks for proper experiment execution, aids in identifying potential problems, and provides solutions to ensure a successful Duolink® PLA experiment for flow cytometry.
General Tips & Tricks
Frequently Asked Questions
This section highlights general considerations and delineates important reminders to help your Duolink® PLA experiment for flow cytometry proceed smoothly.
Figure 1.The Duolink PLA signal is stable. The same cells stored for a week give the same result as those immediately analyzed.
*The excitation laser line represents commonly used lasers that excite the fluorophore. It does not necessarily reflect the lasers available for each particular instrument.
Despite all precautions, sometimes the results are not as expected. Lack of signal in a positive control or high background are two of the most common issues when performing immunodetection experiments, and may also occur with the Duolink® PLA technology. The tables below list the probable causes and suggested solutions to get around these common problems.
Refer to the Duolink® PLA Troubleshooting Guide for a list of frequently asked questions around the Duolink® PLA technology.