These products are available in the US, Canada, and select European countries.
CD34 is a glycosylated transmembrane protein that serves as a well-known marker for primitive blood- and bone marrow-derived progenitor cells, particularly for hematopoietic and endothelial progenitors. Although the biological functions of CD34 are largely unknown, recent data suggest that CD34 is involved in maintenance of the progenitor cells in a phenotypically undifferentiated state.
PromoCell® CD34+ Progenitor Cells are isolated from the cord blood of healthy donors. CD34+ Progenitor Cells are suitable for diverse studies, as well as for the directed differentiation into more committed blood cell phenotypes and endothelial lineages. Immediately after isolation, the freshly prepared CD34+ Progenitor Cells are cryopreserved using proprietary serum-free freezing medium Cryo-SFM. Each cryovial contains at least 100,000 viable cells after thawing.
Hematopoietic Progenitor Cell Expansion Media was developed specifically for the expansion of HPC, and is available in two formulations. These media are provided in kits consisting of a bottle of Basal Medium and one vial of SupplementMix. Adding the SupplementMix to the Basal Medium constitutes the complete medium.
Note: To achieve optimal performance, the complete Hematopoietic Progenitor Cell (HPC) Expansion Medium DXF must be supplemented by the user with additional growth factors. Cytokine Mix E is a ready-to-use mixture of growth factors optionally available for the HPC Expansion Medium DXF. The animal component-free, defined formula contains recombinant human TPO (thrombopoietin), SCF (stem cell factor), flt-3 ligand and IL-3. The optimized formulation allows vigorous expansion of hematopoietic progenitor cells, while maintaining their primitive phenotype and prohibiting differentiation.
The Hematopoietic Progenitor Medium contains all of the growth factors and supplements necessary for healthy expansion of progenitor cells. The HPC Expansion Medium DXF must be supplemented by the user with additional growth factors. Available supplements include the optionally available Cytokine Mix E (C-39890, 1 mL or C-39891, 5 mL) containing recombinant human TPO, SCF, flt-3 ligand, and IL-3. However, the user may customize the medium by supplementation with other growth factors. Hematopoietic Progenitor Media do not contain antibiotics or antimycotics and are formulated for use in an incubator with an atmosphere of 5% CO2.
Important Note: For the Hematopoietic Progenitor Cell (HPC) Expansion Medium DXF, also thaw the Cytokine Mix E at 15 – 25 °C. Aseptically mix the solutions by carefully pipetting up and down. Transfer the entire contents to the corresponding amount of complete Medium. Close the bottle and swirl gently until a homogeneous mixture is achieved. One milliliter of Cytokine Mix E is sufficient for the supplementation of 100 mL HPC Expansion Medium DXF. Alternatively, supplement the HPC Expansion Medium DXF with growth factors of your choice.
Protocol for Cryopreserved Hematopoietic Cells
Rigid quality control tests are performed for each lot of Human CD34+ Progenitor Cells.
* Applicable for CFU-assays for hematopoietic progenitor cells (LTC-IC assays).
All lots of Hematopoietic Progenitor Media are subjected to rigorous quality control tests using primary human mononuclear cells as well as purified hematopoietic progenitor cells from cord blood. Each lot of HPC Expansion Medium DXF is tested for the ability to support the expansion of hematopoietic progenitor cells with a primitive phenotype. Each lot of Hematopoietic Progenitor Medium is tested for the capacity to induce directed differentiation into hematopoietic lineages using purified CD34+ progenitor cells from cord blood. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).
Intended Use for Hematopoietic Progenitor Cells and Media System
These products are for in vitro use only and not for diagnostic or therapeutic procedures. For safety precautions please see appropriate MSDS.