XCell mini-cells are compatible with mPAGE™ gels with the use of mPAGE™ Adapter Plates. Use one Adapter Plate per gel. Position the Adapter Plate against the tall plate of the gel cassette before assembling gel tank (Figure 1).
Figure 1.Use of an mPAGE™ Adapter Plate with an mPAGE™ gel for protein electrophoresis.
There are two buffers that can be used as running buffers for SDS-PAGE gels: MES and MOPS. MES has a lower pKa than MOPS, which enables the gel to run faster. MES buffer gives better separation of proteins at lower molecular weights, while MOPS buffer provides better separation at higher molecular weights (Figure 2).
Figure 2.Selection of MES (left image) vs. MOPS (right image) running buffer for mPAGE™ protein gel electrophoresis, based on protein size and percent acrylamide gel. Protein size for corresponding bands indicated in kDa. Smaller proteins are resolved more efficiently with MES running buffer; larger proteins are resolved more efficiently with MOPS running buffer.
Figure 3.Improper and proper pipette angle for loading samples into mPAGE™ pre-cast gels.
Figure 4.Use the mPAGE™ Gel Cassette Opener as a lever to remove gel plates.