Thin Layer Chromatography Identification Test for Dextromethorphan following European Pharmacopeia Monograph on Silica Gel G 254 Plates


Dextromethorphan is a drug of the morphinan class. It is a cough suppressant with sedative, dissociative, and stimulant properties (at higher doses) and it is the active ingredient in many over-the-counter cold and cough medicines.

This application note illustrate how Silica Gel G plates are suitable for the analysis of Dextromethorphan following the European pharmacopeia identification TLC test experimental conditions (version 9.0).

Dextromethorphan Hydrobromide

Figure 1.Dextromethorphan Hydrobromide

Table 1.Experimental Conditions
TLC plate development

Figure 2.TLC plate development


Figure 3.Chromatograms


Figure 4.Spectrum

Chromatographic Data (Standard Solution)

Figure 5.Chromatographic Data (Standard Solution)

Chromatographic Data (Sample Solution)

Figure 6.Chromatographic Data (Sample Solution)

Table 2.Standard Repeatability
  1. The principal spot in the chromatogram obtained with the test solution is similar in position, colour and size to the principal spot in the chromatogram obtained with reference solution.
  2. All the spots for the reference solution have a repeatability better than 3% RSD.
  3. Merck silica gel G plates provides repeatable and reproducible results for Dextromethorphan identification test and comply to all requirements as per European Pharmacopoeia.