HPLC Analysis of Salicylic Acid and Acetylsalicylic Acid (Aspirin) on Ascentis® Express OH5
Materials
standard
Product No.
Description
Pricing
Salicylic acid
meets analytical specification of Ph. Eur., BP, USP, 99.5-100.5% (calc. to the dried substance)CONDITIONS
column
Ascentis Express OH5, 10 cm x 3.0 mm I.D., 2.7 μm particles (53769-U)
mobile phase
2 mM ammonium acetate in 10:90 water:acetonitrile, pH to 7.0 with acetic acid, glacial
flow rate
0.5 mL/min
pressure
850 psi (59 bar)
column temp.
35 °C
detector
UV, 235 nm
injection
2 μL
sample
100 μg/mL salicylic acid, 200 μg/mL acetylsalicylic acid in methanol:acetonitrile (3:7)
Description
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany