HomeLarge Molecule HPLCHPLC Analysis of a Procainamide-Labeled Dextran Ladder on BIOshell™ Glycan using HILIC-FLR
HPLC Analysis of a Procainamide-Labeled Dextran Ladder on BIOshell™ Glycan using HILIC-FLR
Conditions
| column | HPLC BIOshell Glycan Column, 15 cm x 2.1 mm I.D., 2.7 µm particles (50994-U) |
| mobile phase | A: 50 mM ammonium formate (Product No. 17843) pH 4.4, [ammonium] = 50 mM, pH adjust with formic acid B: Acetonitrile (Product No. 34851) |
| gradient | 75% to 59% B in 75 min |
| flow rate | 0.3 mL/min |
| sample | Dextran Ladder (Product 31417) after Procainamide Labeling Protocol |
| injection | 10 µL |
| detector | FLR (308 nm excitation, 359 emission) |
Description
| Analysis Note | The dextran ladder is used as an external standard for the analysis of glycans by normal phase HPLC after fluorescent labeling. When analyzed on the BIOshell Glycan HPLC Column, this standard gives a characteristic ladder profile from monomeric glucose to approximately a 20-mers of glucose oligosaccharide. This ladder provides calibration reference points that can aide in identifying more complex glycans based upon retention characteristics. |
| Categories | Analytical Chromatography, Proteins, Peptides |
| Featured Industry | Life Science and Biopharma |
| suitability | application for HPLC |
Materials
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