UHPLC/MS Analysis of Diazepam and Metabolites on Titan C18, Comparison of Flow Rate and Column Length
Materials
standard
Product No.
Description
Pricing
Oxazepam solution
1 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Nordiazepam solution
1 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Diazepam solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Temazepam solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®CONDITIONS
column
Titan C18, 2 cm x 2.1 mm I.D., 1.9 μm particles (577120-U)
mobile phase
[A] 0.1% formic acid; [B] acetonitrile; (60:40, A:B)
flow rate
various
temp.
35 °C
detector
TIC MRM (UV also runs in series)
injection
1 μL
sample
100 ng/mL in mobile phase
Description
Analysis Note
These two sets of chromatograms show separations on 100, 50, 30, and 20 mm x 2.1 mm I.D. columns and at different flow rates and demonstrate the utility of the 1.9 μm Titan particles. The highest grade solvents provided clean, robust operation. Cerilliant CRMs provided reliable quantification.
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